Удосконалення методу кількісного підрахунку личинок нематод

Abstract

Background. Helminthes localizing in various tissues and organs, secrete large amounts of eggs and larvae, which mainly come with feces in the environment. With a view to the selection of pathogens and helminthes helmintolarvascopy offered a significant amount of diagnostics methods, mostly based on the detection of larvae without quantitative calculation. Objective. The aim of research was to improve the methods of quantitative calculation nematode larvae and compared the proposed method with well-known. Methods. The study was conducted in laboratory conditions, Department of Parasitology and Veterinary Expertise Poltava State Agrarian Academy and Dnepropetrovsk State Agrarian and Economic University. The material for the research served was fecal horses spontaneously affected by pathogens helminthes. Cultivation of larvae Strogylus spp. and Strongyloides westeri carried by method of Velychkin. In the first phase of research conducted for the selection of larvae generally helmintolarvascopy by Berman. For a quantitative calculation larvae used method by Orlov and improved method. When comparing the diagnostic methods take into account the number of larvae isolated in 1 g of feces, ease of research and time spent on their conduct. Results. The proposed quantitative method of counting the larvae of nematodes is to use a special counting grid marked on the bottom of a petri dish, consisting of 5 squares 1×1 cm. Inside squares divided into 5 equal parallel track length of 10 mm, a width of 0.2 mm. The size of the tracks specially selected to increase by microscope × 40 or × 100, looked through their land, which makes it easier to count the number of larvae. The effectiveness of the proposed method was confirmed in laboratory conditions, conducting its comparison with the prototype method by I. Orlov. For this purpose a study of 30 samples of feces of horses. The proposed method found more larvae (at 13.78%). However, there are simple count of the number of larvae in 1 g of feces. For added convenience, constantly using petri dishes of the same diameter, which makes it possible to reduce the number of repetitive calculations. Conclusion. The proposed method of counting the number of larvae Strogylus spp. and Strongyloides westeri in 1 g of feces using a counting grid deposited on the bottom of a petri dish exceeds the known methods for the efficient determination of the intensity of infestation, ease of implementation, that allows us to recommend an improved method for to using in practical laboratory diagnostic by mass parasitological helmintolarvascopy researches.Представлено удосконалений метод кількісного підрахунку личинок нематод з використанням лічильної сітки, нанесеної на дно чашки Петрі. Визначено ефективність запропонованого способу порівняно із загальноприйнятою методикою за В. І. Орловим. Встановлено високу діагностичну ефективність удосконаленого кількісного методу за стронгілоїдозу та стронгілідозів коней.