Вплив препарату 5-тієніл-1,2,4-триазол-3-тіону на морфологію, тинкторіальні та культуральні властивості патогенних та дисоціативних форм Mycobacterium bovis

Abstract

Показано вплив 5-тієніл-1,2,4-триазол-3-тіону у трьох концентраціях (0,1; 0,5; 1%) на морфологічні ознаки, тинкторіальні та культуральні властивості патогенних і дисоціативних форм мікобактерій. Встановлено, що препарат володіє бактеріостатичною дією за різних концентрацій, щодо патогенного штаму M. bovis, культивованого на живильному середовищі з рН 7,1 та 6,5 за температури 37 °С та дисоціативних форм мікобактерій 117а пересіву, які культивувалися за 3 °С і 1 % концентрації препарату. Проте в концентрації 0,1 та 0,5 % згаданий препарат не призупинив розмноження дисоціативних форм мікобактерій та відповідно ріст колоній на живильних середовищах з рН 7,1 та 6,5; бактеріоскопією культур встановили некислотостійкі палички різної довжини, кокоподібні та поодинокі овалоподібні (L-) форми рівномірно зафарбовані та із зернами. У поодиноких некислотостійких зернистих паличок виявлені кислотостійкі зерна на одному чи двох полюсах. One of forms of the variability of tuberculosis mycobacteria - the formation of resistance to antibiotics. It is associated with uncontrolled and irrational usage for treatment, both among people and animals. Mycobacterium acquire the ability to exist and reproduce at a concentration of antibacterial drugs, which are usually incompatible with their life. Therefore, the actual tasks of researchers in veterinary and humane medicine are a detailed study of properties acquired by mycobacteria in order to find new and more modern means of prevention and treatment of this disease. The aim of this investigation was to study the morphology, cultural and tinctorial properties of pathogenic mycobacteria and dissociative forms that were cultured through a nutrient medium with the addition of 5-thienyl-1,2,4-triazole-3-thione (SKP-123) at three concentrations (0.1; 0.5; 1%). The results of the investigation have been shown that the drug involved at different concentrations (0.1; 0.5; 1%) inhibits the growth and development of pathogenic strain of M. bovis, cultured through media with pH 7.1 and 6.5 at 37 °C and dissociative forms of mycobacteria of 117a subculture that were cultured at 3 °C. There was no growth of the colonies of this strain of mycobacteria at 1% concentration of the drug for 90 days, that indicates its bacteriastatic effect. Microscopy of smears made by "blind" method from medium without growth of culture has found residual forms of mycobacteria: from pathogenic - acid-proof bacilli of different length, as well as acid-proof grains, and from dissociative forms - acid-nonproof short bacillus, coccoid acid-nonproof forms, non-uniformly painted single ovaloid (L-) acid-proof forms. It has been observed that SKP-123 at concentrations of 0.1 and 0.5% did not suspend the reproduction of dissociative forms of mycobacteria and colony growth through nutrient media with pH 7.1 and 6.5, аcid-nonproof bacilli of different length, acid-proof coccoid and uniformly painted ovaloid acid-proof (L-) forms and with grains. Single acid-proof bacilli revealed acid-proof grains on one or two poles were established by the microscopy of cultures. The growth of culture of 100th passage of M. bovis through a medium with pH 7.1 at 37 °C as a rough plaque has been observed in the control group starting from 7th day, and after 4 days on a medium with a pH 6.5 small colonies among the sowing line have been observed. Аcid-proof short bacilli and grains were observed by smear microscopy of M. bovis, that have been grown through a nutrient medium with pH 7.1; acid-proof rods with different length and grains were observed by smear microscopy of M. bovis that have been grown through a nutrient medium with pH 6.5. The culture growth in the control group of dissociative forms of 117a variant of M. bovis through the medium with pH 7.1 and 6.5 at 3 °C was observed since the third day of the experiment and was characterized by slimy film with gray, then gray-yellow color and furthermore, solid growth with orange color. Smear microscopy of dissociative forms (control), that have been grown through a nutrient medium with pH 7.1 showed single or in chains acid-proof bacillus, large oval forms with acid-proof grains inside; acid-proof small coccoid forms without grains in small amounts, single acid-proof elementary bodies; through a medium with pH 6.5 - acid-proof bacilli with different length, that housed both single and in clusters, coccoid and acid-proof and acid-nonproof grains. Also ovaloid (L-) forms that were several times larger than the bacilli, and unevenly dyed with acid-proof grains at the center. At the same time, the given study requires further investigation on the effect of the drug 5-thienyl1,2,4-triazole-3-tion to mycobacteria in vivo.